NFYB Transcription Factor Activity Assay
- SKU:
- TFAB00188
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- NFYB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
Description
| Product Name: | NFYB Transcription Factor Activity Assay |
| Product Code: | TFAB00188 |
| Target: | NFYB |
| Synonyms: | CAAT box transcription factor activity protein subunit B, Nuclear transcription factor Y subunit B, NF-YB |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie NFYB transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 12q22-q23 Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus Molecular Function:protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process |
| NCBI Summary: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25208 |
| NCBI GenInfo Identifier: | 399193 |
| NCBI Gene ID: | 4801 |
| NCBI Accession: | P25208.2 |
| UniProt Secondary Accession: | P25208,Q96IY8, A8K7B9, |
| UniProt Related Accession: | P25208 |
| Molecular Weight: | 207 |
| NCBI Full Name: | Nuclear transcription factor Y subunit beta |
| NCBI Synonym Full Names: | nuclear transcription factor Y, beta |
| NCBI Official Symbol: | NFYB |
| NCBI Official Synonym Symbols: | HAP3; CBF-A; CBF-B; NF-YB |
| NCBI Protein Information: | nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A |
| UniProt Protein Name: | Nuclear transcription factor Y subunit beta |
| UniProt Synonym Protein Names: | CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB |
| Protein Family: | Nuclear transcription factor |
| UniProt Gene Name: | NFYB |
| UniProt Entry Name: | NFYB_HUMAN |
