NFkB Phosphorylation Assay Kit (Fluorometric) (BA0136)

NFkB Phosphorylation Assay Kit

Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-alpha. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription

Assay Genie's cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.

Applications

For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.

Key Features

• Simple and convenient. No cell lysis necessary, cells can be cultured for several days.

NFkB Phosphorylation Assay Kit Information:

Kit Includes:	10x Wash Buffer: 25 mL, Blocking Buffer: 25 mL, Protein Stain: 6 mL, HRP Substrate: 6 mL, pNF-kappaB-Ab1: 10 uL HRP-Ab2 10 uL
Kit Requires:	37% formaldehyde; 3% H2O2 ; black cell culture 96-well plate; plate sealers; deionized or distilled water; pipetting devices; cell culture incubators; centrifuge tubes; fluorescence plate reader capable of reading at ex/em =530/585 nm and at ex/em =360/450 nm
Method of Detection:	FL360/450nm, 530/585nm
Samples:	Cell, tissue etc
Species:	Human, Mouse
Protocol Length:	5hrs
Size:	100 tests
Storage:	Store all reagents at -20°C
Shelf Life:	6 months