NETO1 Antibody (PACO58348)
- SKU:
- PACO58348
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | NETO1 Antibody (PACO58348) |
| Antibody SKU: | PACO58348 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Neuropilin and tolloid-like protein 1 protein (367-507AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, U87 whole cell lysate. All lanes: NETO1 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 61, 18 kDa. Observed band size: 61 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO58348 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory (By similarity). |
| Synonyms: | Neuropilin and tolloid-like protein 1 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1), NETO1, BTCL1 |
| UniProt Protein Function: | NETO1: a transmembrane protein and component of the N-methyl-D-aspartate receptor (NMDAR) complex. Required to maintain the normal level of NMDAR subunits at synapses. The NMDAR complex is a major excitatory ligand-gated ion channel in the central nervous system and a major mediator of synaptic plasticity. Important in the development and/or maintenance of neuronal circuitry. Neto1-deficient mice have a dramatic decrease in the number of NMDA receptors at synapses, impairing learning and synaptic plasticity. Three isoforms of the human protein are produced by alternative splicing. Isoforms 1 and 2 are retina-specific whereas isoform 3 is found in retina as well as at lower levels in adult and fetal brain.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 18q22.2Cellular Component: postsynaptic membrane; kainate selective glutamate receptor complex; postsynaptic density; extracellular region; excitatory synapse; cell junctionMolecular Function: ionotropic glutamate receptor bindingBiological Process: regulation of long-term neuronal synaptic plasticity; visual learning; memory |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in mice encodes a protein that plays a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | Q8TDF5 |
| NCBI GenInfo Identifier: | 319918840 |
| NCBI Gene ID: | 81832 |
| NCBI Accession: | NP_001188394.1 |
| UniProt Secondary Accession: | Q8TDF5,Q86W85, Q8ND78, Q8TDF4 |
| UniProt Related Accession: | Q8TDF5 |
| Molecular Weight: | 60,060 Da |
| NCBI Full Name: | neuropilin and tolloid-like protein 1 isoform 3 |
| NCBI Synonym Full Names: | neuropilin (NRP) and tolloid (TLL)-like 1 |
| NCBI Official Symbol: | NETO1 |
| NCBI Official Synonym Symbols: | BCTL1; BTCL1 |
| NCBI Protein Information: | neuropilin and tolloid-like protein 1; brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
| UniProt Protein Name: | Neuropilin and tolloid-like protein 1 |
| UniProt Synonym Protein Names: | Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
| Protein Family: | |
| UniProt Gene Name: | NETO1 |
| UniProt Entry Name: | NETO1_HUMAN |
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