NEK10 Antibody (PACO62491)
- SKU:
- PACO62491
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | NEK10 Antibody (PACO62491) |
| Antibody SKU: | PACO62491 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Serine/threonine-protein kinase Nek10 protein (1-104AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO62491 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO62491 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | protein kinase complex, protein kinase activity, positive regulation of MAP kinase activity, positive regulation of protein autophosphorylation, protein phosphorylation, regulation of cell cycle G2/M phase transition, regulation of ERK1 and ERK2 cascade |
| Synonyms: | Serine/threonine-protein kinase Nek10 (EC 2.7.11.1) (Never in mitosis A-related kinase 10) (NimA-related protein kinase 10), NEK10 |
| UniProt Protein Function: | NEK10: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NEK familyChromosomal Location of Human Ortholog: 3p24.1Cellular Component: centrosome; cytoplasm; nucleusMolecular Function: protein kinase activityBiological Process: chromosome segregation; microtubule-based process; positive regulation of MAP kinase activity; positive regulation of protein amino acid autophosphorylation; protein amino acid phosphorylation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q6ZWH5 |
| NCBI GenInfo Identifier: | 380865464 |
| NCBI Gene ID: | 152110 |
| NCBI Accession: | Q6ZWH5.3 |
| UniProt Secondary Accession: | Q6ZWH5,Q45VJ4, Q6ZR11, Q7Z671, Q86XB1, Q96MB3, A8MWG1 B9ZVR0 |
| UniProt Related Accession: | Q6ZWH5 |
| Molecular Weight: | 47,765 Da |
| NCBI Full Name: | Serine/threonine-protein kinase Nek10 |
| NCBI Synonym Full Names: | NIMA related kinase 10 |
| NCBI Official Symbol: | NEK10 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | serine/threonine-protein kinase Nek10 |
| UniProt Protein Name: | Serine/threonine-protein kinase Nek10 |
| UniProt Synonym Protein Names: | Never in mitosis A-related kinase 10; NimA-related protein kinase 10 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | NEK10 |
| UniProt Entry Name: | NEK10_HUMAN |
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