NEBL Antibody (PACO59812)
- SKU:
- PACO59812
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | NEBL Antibody (PACO59812) |
| Antibody SKU: | PACO59812 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Nebulette protein (438-533AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of PC-3 cells with PACO59812 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion. |
| Synonyms: | Nebulette (Actin-binding Z-disk protein), NEBL, LNEBL |
| UniProt Code: | O76041 |
| NCBI GenInfo Identifier: | 291190154 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_001166955.1 |
| Molecular Weight: | Predicted Molecular Mass: 60.3kDaAccurate Molecular Mass: 56kDa |
| NCBI Full Name: | nebulette isoform 3 |
| UniProt Protein Name: | Nebulette |
| UniProt Synonym Protein Names: | Actin-binding Z-disk protein |
| Protein Family: | Nebulette |
| UniProt Gene Name: | NEBL |
| UniProt Entry Name: | NEBL_HUMAN |
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