Name: NDUFB6 Antibody (PACO28534)
SKU: PACO28534
Availability: OutOfStock

Antibody Name:	NDUFB6 Antibody (PACO28534)
Antibody SKU:	PACO28534
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 6 protein (2-67AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot Positive WB detected in: HEK293 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue, Mouse thymus tissue All lanes: NDUFB6 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16, 14 kDa Observed band size: 16, 14 kDa .
	Immunoprecipitating NDUFB6 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of (1µg) instead of PACO28534 in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PACO28534 (4µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg) .
	Immunohistochemistry of paraffin-embedded human lung cancer using PACO28534 at dilution of 1:100.

Background:	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Synonyms:	NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 6 (Complex I-B17) (CI-B17) (NADH-ubiquinone oxidoreductase B17 subunit), NDUFB6

UniProt Protein Function:	NDUFB6: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB6 subunit family.Protein type: Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Oxidoreductase; Membrane protein, integral; EC 1.6.99.3; MitochondrialChromosomal Location of Human Ortholog: 9p21.1Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; mitochondrial respiratory chain complex IMolecular Function: NADH dehydrogenase (ubiquinone) activityBiological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jan 2011]
UniProt Code:	O95139
NCBI GenInfo Identifier:	6093474
NCBI Gene ID:	4712
NCBI Accession:	O95139.3
UniProt Secondary Accession:	O95139
UniProt Related Accession:	O95139
Molecular Weight:
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit B6
NCBI Official Symbol:	NDUFB6
NCBI Official Synonym Symbols:	CI; B17
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
UniProt Synonym Protein Names:	Complex I-B17; CI-B17; NADH-ubiquinone oxidoreductase B17 subunit
Protein Family:
UniProt Gene Name:	NDUFB6
UniProt Entry Name:	NDUB6_HUMAN