Antibody Name:	NCDN Antibody (PACO57880)
Antibody SKU:	PACO57880
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Neurochondrin protein (403-516AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissue. All lanes: NCDN antibody at 2.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 79, 78, 80 kDa. Observed band size: 79 kDa.
	Immunofluorescence staining of HepG2 cells with PACO57880 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO57880 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Probably involved in signal transduction, in the nervous system, via increasing cell surface localization of GRM5 and positively regulating its signaling (By similarity). Required for the spatial learning process. Acts as a negative regulator of Ca(2+)-calmodulin-dependent protein kinase 2 (CaMK2) phosphorylation. May play a role in modulating melanin-concentrating hormone-mediated functions via its interaction with MCHR1 that interferes with G protein-coupled signal transduction. May be involved in bone metabolism. May also be involved in neurite outgrowth.

Synonyms:

Neurochondrin, NCDN, KIAA0607

UniProt Protein Function:	NCDN: Probably involved in signal transduction, in the nervous system, via increasing cell surface localization of GRM5 and positively regulating its signaling. Required for the spatial learning process. Acts as a negative regulator of Ca(2+)-calmodulin-dependent protein kinase 2 (CaMK2) phosphorylation. May play a role in modulating melanin- concentrating hormone-mediated functions via its interaction with MCHR1 that interferes with G protein-coupled signal transduction. May be involved in bone metabolism. May also be involved in neurite outgrowth. Belongs to the neurochondrin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: axon; cell soma; cytosol; dendrite; membrane; nucleus Molecular Function:protein binding Biological Process: neurite development
NCBI Summary:	This gene encodes a leucine-rich cytoplasmic protein, which is highly similar to a mouse protein that negatively regulates Ca/calmodulin-dependent protein kinase II phosphorylation and may be essential for spatial learning processes. Several alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UBB6
NCBI GenInfo Identifier:	74753219
NCBI Gene ID:	23154
NCBI Accession:	Q9UBB6.1
UniProt Secondary Accession:	Q9UBB6,Q9UBY2, Q9Y4A6, Q9Y4D9, D3DPR9,
UniProt Related Accession:	Q9UBB6
Molecular Weight:	79,158 Da
NCBI Full Name:	Neurochondrin
NCBI Synonym Full Names:	neurochondrin
NCBI Official Symbol:	NCDN
NCBI Protein Information:	neurochondrin
UniProt Protein Name:	Neurochondrin
Protein Family:	Neurochondrin
UniProt Gene Name:	NCDN
UniProt Entry Name:	NCDN_HUMAN