NAGPA Antibody (PACO59804)
- SKU:
- PACO59804
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | NAGPA Antibody (PACO59804) |
| Antibody SKU: | PACO59804 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase protein (327-438AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: NAGPA antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 53, 34 kDa. Observed band size: 53 kDa. |
 | IHC image of PACO59804 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases. |
| Synonyms: | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (EC 3.1.4.45) (Mannose 6-phosphate-uncovering enzyme) (Phosphodiester alpha-GlcNAcase), NAGPA |
| UniProt Protein Function: | Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases. |
| UniProt Protein Details: | |
| NCBI Summary: | Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015] |
| UniProt Code: | Q9UK23 |
| NCBI GenInfo Identifier: | 66346700 |
| NCBI Gene ID: | 51172 |
| NCBI Accession: | NP_057340.2 |
| UniProt Secondary Accession: | Q9UK23,Q96EJ8, B2RAS1 |
| UniProt Related Accession: | Q9UK23 |
| Molecular Weight: | 33,577 Da |
| NCBI Full Name: | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase |
| NCBI Synonym Full Names: | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase |
| NCBI Official Symbol: | NAGPA |
| NCBI Official Synonym Symbols: | UCE; APAA |
| NCBI Protein Information: | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase |
| UniProt Protein Name: | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase |
| UniProt Synonym Protein Names: | Mannose 6-phosphate-uncovering enzyme; Phosphodiester alpha-GlcNAcase |
| Protein Family: | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase |
| UniProt Gene Name: | NAGPA |
| UniProt Entry Name: |
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