Metabolism Assays

NAD/NADH Assay Kit (Colorimetric) (BA0067)

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SKU:: BA0067
UPC:
MPN:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Cell Or Tissue Extracts
Research Area:: Glycolysis & Carbohydrates
Research Area:: Urea
Research Area:: Oxidative Stress
Research Area:: Antioxidant
Research Area:: Diabetes & Obesity

Description
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Description

NAD/NADH Assay Kit - Information

Assay Genie's NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD/NADH concentration in the sample. This assay is highly specific for NAD⁺/NADH and with minimal interference (<1%) by NADP⁺/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.

Applications

For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.

NAD/NADH Assay Kit - Key Features

Sensitive and accurate. Detection limit of 0.05 uM and linearity up to 10 uM NAD+/NADH in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

NAD/NADH Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL Enzyme A: 120 mL Lactate: 1.5 mL Enzyme B: 120 mL MTT Solution: 1.5 mL NAD Standard: 0.5 mL NAD/NADH Extraction Buffers: each 12 mL
Kit Requires	Pipetting (multi-channel) devices. Clear-bottom 96-well plates and plate reader.
Method of Detection	OD565nm
Detection Limit	0.05 uM
Samples	Cell or tissue extracts
Species	All
Protocol Length	15 min
Size	100 tests
Storage	Store all reagents at -20°C.
Shelf Life	6 months

More Details

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD⁺/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD⁺/NADH concentration are very desirable.

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