Description
Product Name: | NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit |
Product Code: | MAES0226 |
Sample Type: | Serum (Plasma), Animal Tissue, Cell |
Assay Time: | 30 min |
Assay Type: | Enzyme Activity |
Synonyms: | NAD-MDH |
Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 1.06 U/L |
Detection Range: | 1.06–46.7 U/L |
Inter-assay CV: | 0.084 |
Intra-assay CV: | 0.03 |
NAD-MDH catalyzes the conversion of substrate malic acid and NAD to oxaloacetic acid and NADH. NADH makes WST-8 orange under the action of electron coupling agent. The activity of NAD-MDH can be calculated by measuring the change of absorbance value at 450 nm.
Storage: | -20°C |
Valid Period: | 12 months |
Other Instruments Required: | Incubator, centrifuge |