Description
| Product Name: | Myelin Basic Protein/MBP Polyclonal Antibody |
| Product Code: | CAB24860 |
| Reactivity: | Mouse, Rat |
| Applications: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Reactivity: | Mouse, Rat |
| Tested Applications: | WB, IHC-P, IF/ICC |
| Key Applications: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500-1:1000, IHC-P 1:50-1:200, IF/ICC 1:50-1:200 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Positive Samples: | Mouse brain |
| Cellular Location: | Cytoplasmic side, Myelin membrane, Nucleus, Peripheral membrane protein |
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human Myelin Basic Protein/MBP (NP_001020272.1). |
| Sequence: | MGNHA GKREL NAEKA STNSE TNRGE SEKKR NLGEL SRTTS EDNEV FGEAD ANQNN GTSSQ DTAVT DSKRT ADPKN AWQDA HPADP GSRPH LIRLF SRDAP GREDN TFKDR PSESD ELQTI QEDSA ATSES LDVMA SQKRP SQRHG SKYLA TASTM DHARH GFLPR HRDTG ILDSI GRFFG GDRGA PKRGS GKDSH HPART AHYGS LPQKS HGRTQ DENPV VHFFK NIVTP RTPPP SQGKG RGLSL SRFSW GAEGQ RPGFG YGGRA SDYKS AHKGF KGVDA QGTLS KIFKL GGRDS RSGSP MARR |
| Synonyms: | MBP, myelin basic protein |
| Calculated MW: | 17kDa/18kDa/20kDa/21kDa/33kDa |
| Observed MW: | 17-33kDa |
