Name: MX2 Antibody (PACO57872)
SKU: PACO57872
Availability: OutOfStock

Antibody Name:	MX2 Antibody (PACO57872)
Antibody SKU:	PACO57872
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Interferon-induced GTP-binding protein Mx2 protein (548-688AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO57872 diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with PACO57872 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.

Synonyms:

Interferon-induced GTP-binding protein Mx2 (Interferon-regulated resistance GTP-binding protein MxB) (Myxovirus resistance protein 2) (p78-related protein), MX2

UniProt Protein Function:	MX2: has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]Protein type: G protein regulator, misc.Chromosomal Location of Human Ortholog: 21q22.3Cellular Component: cytoplasm; nuclear pore; cytosol; nucleusMolecular Function: GTPase activity; GTP bindingBiological Process: protein transport; mRNA transport; metabolic process; regulation of cell cycle; regulation of nucleocytoplasmic transport; cytokine and chemokine mediated signaling pathway; response to virus; defense response; defense response to virus
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]
UniProt Code:	P20592
NCBI GenInfo Identifier:	11342664
NCBI Gene ID:	4600
NCBI Accession:	NP_002454.1
UniProt Secondary Accession:	P20592,B7Z5D3, D3DSI7
UniProt Related Accession:	P20592
Molecular Weight:
NCBI Full Name:	interferon-induced GTP-binding protein Mx2
NCBI Synonym Full Names:	MX dynamin-like GTPase 2
NCBI Official Symbol:	MX2
NCBI Official Synonym Symbols:	MXB
NCBI Protein Information:	interferon-induced GTP-binding protein Mx2; p78-related protein; myxovirus resistance protein 2; second interferon-induced protein p78; myxovirus (influenza virus) resistance 2; interferon-regulated resistance GTP-binding protein MXB
UniProt Protein Name:	Interferon-induced GTP-binding protein Mx2
UniProt Synonym Protein Names:	Interferon-regulated resistance GTP-binding protein MxB; Myxovirus resistance protein 2; p78-related protein
Protein Family:	Interferon-induced GTP-binding protein
UniProt Gene Name:	MX2
UniProt Entry Name:	MX2_HUMAN