Antibody Name:	MUC1 Antibody (PACO20981)
Antibody SKU:	PACO20981
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:	Human, Mouse
Immunogen:	Synthetic peptide of human MUC1(CT)

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20981(MUC1(CT) Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20981(MUC1(CT) Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into α and β subunits that form a heterodimeric complex. The N-terminal α subunit functions in cell-adhesion and the C-terminal β subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Synonyms:

mucin 1, cell surface associated

UniProt Protein Function:	MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Actin-binding; Membrane protein, integral; Tumor suppressor; Cell adhesion Chromosomal Location of Human Ortholog: 1q21 Cellular Component: extracellular space; cell surface; integral to plasma membrane; Golgi lumen; cytoplasm; nuclear chromatin; apical plasma membrane; vesicle Molecular Function:protein binding; p53 binding; transcription cofactor activity Biological Process: protein amino acid O-linked glycosylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; cellular protein metabolic process; regulation of transcription from RNA polymerase II promoter in response to stress; O-glycan processing; post-translational protein modification; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Disease: Medullary Cystic Kidney Disease 1
NCBI Summary:	This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
UniProt Code:	P15941
NCBI GenInfo Identifier:	296439295
NCBI Gene ID:	4582
NCBI Accession:	P15941.3
UniProt Secondary Accession:	P15941,P13931, P15942, P17626, A5YRV1, A6ZID9, A6ZIE0 B1AVQ8, B1AVR0, B6ECA1, E7ESE5, E7EUG9,
UniProt Related Accession:	P15941
Molecular Weight:
NCBI Full Name:	Mucin-1
NCBI Synonym Full Names:	mucin 1, cell surface associated
NCBI Official Symbol:	MUC1
NCBI Official Synonym Symbols:	EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
NCBI Protein Information:	mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant
UniProt Protein Name:	Mucin-1
UniProt Synonym Protein Names:	Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT
Protein Family:	Mucin
UniProt Gene Name:	MUC1
UniProt Entry Name:	MUC1_HUMAN

Antibodies	ELISA Kits
Anti-MUC1 Antibody (CAB0333)	Human Mucin-1 / MUC1 ELISA Kit
Anti-MUC1 Antibody (CAB19081)	Human MUC1 (Mucin 1) CLIA Kit (HUES00408)
MUC1 Antibody (PACO01119)	Human MUC1 (Mucin 1) ELISA Kit (HUES01780)
Phospho-MUC1 (Y1229) Antibody (PACO04364)	Human Mucin-1 (MUC1) ELISA Kit
Phospho-MUC1 (Y1243) Antibody (PACO04365)