Cell Health Assays
MTT Cell Viability Assay (BA0004)
- SKU:
- BA0004
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Cell Culture
- Research Area:
- Cell Viability & Proliferation
- Research Area:
- High-Throughput Screening
Description
| Product Name: | MTT Cell Viability Assay Kit |
| Product Code: | BA0004 |
| Reactivity: | General |
| Size: | 500 tests |
| Sample Type: | Cell culture |
| Detection Limit: | 950 cells |
| Detection Method: | OD 570nm |
| Protocol Length: | 5 hours |
| Applications: | Cell viability, proliferation, cytotoxicity and high-throughput screening for anti-cancer agents |
| Storage: | The kit is shipped at room temperature. Store the Reagent at -20°C. Solubilization Solution can be stored at room temperature |
| Shelf life: | 12 months |
| Note: | For research use only |
The study of cell proliferation and cell viability has been a key area of research in the life sciences, including areas such as drug discovery and development. The MTT cell viability assay is a traditional cell proliferation assay that measures the activity of mitochondrial enzymes responsible for conversion of tetrazolium dye MTT to formazan product by viable cells. This cell-based assay can be performed in multi-well plates and is compatible with all culture media. The assay is also amenable to high-throughput screening applications in 96-well and 384-well plates.
The MTT assay has been used to measure cell proliferation, cytotoxicity and apoptosis in a wide variety of cell types, including mammalian cells, yeast cells, plant cells and bacteria cells. The Assay Genie MTT cell viability assay has been optimized for maximum sensitivity, reproducibility and long shelf-life.
| Safe: | Non-radioactive assay (cf. 3H-thymidine incorporation assay) |
| Sensitive and accurate: | As low as 950 cells can be accurately quantified |
| Time efficient: | High-throughput assay in 96-well allows simultaneous processing ten of thousands of samples per day |
| Homogeneous and convenient: | A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved |
| Robust and amenable to HTS: | Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems |
| Nurcahyanti et al. | Bixin and fucoxanthin sensitize human lung cancer and cervical cancer cell to cisplatin in vitro | BMC Res Notes. 2021 | PubMed ID: 34922615 |
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