MTRF1 Antibody (PACO49786)
- SKU:
- PACO49786
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MTRF1 Antibody (PACO49786) |
| Antibody SKU: | PACO49786 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Peptide chain release factor 1, mitochondrial protein (285-433AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human liver cancer using PACO49786 at dilution of 1:100. |
 | Immunofluorescence staining of Hela cells with PACO49786 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: MTRF1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 55 kDa. Observed band size: 53 kDa. |
| Background: | Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA. |
| Synonyms: | Peptide chain release factor 1, mitochondrial (MRF-1) (MtRF-1), MTRF1 |
| UniProt Code: | O75570 |
| NCBI GenInfo Identifier: | 62512140 |
| NCBI Gene ID: | 9617 |
| NCBI Accession: | O75570.2 |
| UniProt Secondary Accession: | O75570,Q5T6Y5, Q8IUQ6, B4DG01, |
| UniProt Related Accession: | O75570 |
| Molecular Weight: | 445 |
| NCBI Full Name: | Peptide chain release factor 1, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial translational release factor 1 |
| NCBI Official Symbol: | MTRF1 |
| NCBI Official Synonym Symbols: | RF1; MRF1; MTTRF1 |
| NCBI Protein Information: | peptide chain release factor 1, mitochondrial; MRF-1; mtRF-1; mitochontrial peptide chain release factor 1 |
| UniProt Protein Name: | Peptide chain release factor 1, mitochondrial |
| UniProt Gene Name: | MTRF1 |
| UniProt Entry Name: | RF1M_HUMAN |
| Antibodies |
| MTRF1 Antibody (PACO10653) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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