Antibody Name:	MOV10L1 Antibody (PACO59401)
Antibody SKU:	PACO59401
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human RNA helicase Mov10l1 protein (336-425AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate. All lanes: MOV10L1 antibody at 6.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 136, 38, 14, 130, 131 kDa. Observed band size: 136 kDa.
	IHC image of PACO59401 diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO59401 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the primary piRNA metabolic process. Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Acts via its ATP-dependent RNA helicase activity: displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins.

Synonyms:

RNA helicase Mov10l1 (EC 3.6.4.13) (Moloney leukemia virus 10-like protein 1) (MOV10-like protein 1), MOV10L1

UniProt Protein Function:	MOV10L1: Putative RNA helicase. Isoform 1 may play a role in male germ cell development. Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Helicase; EC 3.6.4.13Chromosomal Location of Human Ortholog: 22q13.33Cellular Component: intracellularMolecular Function: RNA binding; magnesium ion binding; ATP binding; ATP-dependent RNA helicase activityBiological Process: multicellular organismal development; spermatogenesis; gene expression; germ cell development
UniProt Protein Details:
NCBI Summary:	This gene is similar to a mouse gene that encodes a putative RNA helicase and shows testis-specific expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
UniProt Code:	Q9BXT6
NCBI GenInfo Identifier:	255759906
NCBI Gene ID:	54456
NCBI Accession:	NP_001157576.1
UniProt Secondary Accession:	Q9BXT6,Q5TGD5, Q8NBD4, Q9NXW3, Q9UFB3, Q9UGX9, A7E211 A8MXC6, B7WPP1, B7Z7R1
UniProt Related Accession:	Q9BXT6
Molecular Weight:	135,293 Da
NCBI Full Name:	putative helicase Mov10l1 isoform 2
NCBI Synonym Full Names:	Mov10l1, Moloney leukemia virus 10-like 1, homolog (mouse)
NCBI Official Symbol:	MOV10L1
NCBI Official Synonym Symbols:	CHAMP; DJ402G11.8
NCBI Protein Information:	putative helicase Mov10l1; Mov10-like 1; MOV10-like protein 1; moloney leukemia virus 10-like protein 1; cardiac helicase activated by MEF2C protein
UniProt Protein Name:	Putative helicase Mov10l1
UniProt Synonym Protein Names:	Moloney leukemia virus 10-like protein 1
Protein Family:
UniProt Gene Name:	MOV10L1
UniProt Entry Name:	M10L1_HUMAN