Cytokines Recombinant Proteins

Mouse TPO Recombinant Protein (RPPB1053)

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RPPB1053

Description

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Product Name:Mouse TPO Recombinant Protein
Product Code:RPPB1053
Size:10µg
Species:Mouse
Target:TPO
Synonyms:Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1.
Source:HEK293 Cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:TPO protein (0.5mg/ml) contains �Phosphate-Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ?4ng/ml.
Amino Acid Sequence:SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HGPVNGTHGL FAGTSLQTLE ASDISPGAFN KGSLAFNLQG GLPPSPSLAP DGHTPFPPSP ALPTTHGSPP QLHPLFPDPS TTMPNSTAPH PVTMYPHPRN LSQETHHHHH H
Biological Activity:Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is ? 25ug/ml.�

Thrombopoietin or TPO or MGDF, is a protein (glycoprotein hormone) that can be found in the liver and kidney tissues. TPO regulates the creation of platelets. The protein enhances the production & differentiation of cells such as megakaryocytes that are part of the bone marrow cells that secretes a wide number of platelets. The cellular development process that ends up in the production of platelet calls (Megakaryocytopoiesis). Humoral growth factor is needed for the megakaryocyte proliferation & maturation of megakaryocyte, not apart from thrombopoiesis.

TPO Mouse Recombinant produced in HEK293 cells is a single, non-glycosylated polypeptide chain containing �341 amino acids ( 22-356 a.a) and having a molecular mass of �36.4kDa. TPO is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.�

UniProt Protein Function:THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Cellular Component: extracellular space; extracellular region

Molecular Function:hormone activity; cytokine activity; receptor binding

Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation

NCBI Summary:This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
UniProt Code:P40226
NCBI GenInfo Identifier:730983
NCBI Gene ID:21832
NCBI Accession:P40226.1
UniProt Related Accession:P40226,JC4227
Molecular Weight:37,384 Da
NCBI Full Name:Thrombopoietin
NCBI Synonym Full Names:thrombopoietin
NCBI Official Symbol:Thpo��
NCBI Official Synonym Symbols:Ml; Tpo; Mgdf; Mpllg��
NCBI Protein Information:thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
UniProt Protein Name:Thrombopoietin
UniProt Synonym Protein Names:C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Protein Family:Thyroid peroxidase
UniProt Gene Name:Thpo��
UniProt Entry Name:TPO_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P40226
Research Area:
Cytokines
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