Cytokines Recombinant Proteins

Mouse TPO Recombinant Protein (RPPB1052)

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RPPB1052

Description

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Product Name:Mouse TPO Recombinant Protein
Product Code:RPPB1052
Size:10µg
Species:Mouse
Target:TPO
Synonyms:Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility:It is recommended to reconstitute the lyophilized Thrombopoietin in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO Mouse should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
Biological Activity:ED50 range is less than 0.8ng/ml as determined by the dose dependent stimulation of Mo7e cells.

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidneyt hat regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.

Thrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton. The TPO is purified by proprietary chromatographic techniques.

UniProt Protein Function:THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Cellular Component: extracellular space; extracellular region

Molecular Function:hormone activity; cytokine activity; receptor binding

Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation

NCBI Summary:This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
UniProt Code:P40226
NCBI GenInfo Identifier:730983
NCBI Gene ID:21832
NCBI Accession:P40226.1
UniProt Related Accession:P40226,JC4227
Molecular Weight:37,384 Da
NCBI Full Name:Thrombopoietin
NCBI Synonym Full Names:thrombopoietin
NCBI Official Symbol:Thpo��
NCBI Official Synonym Symbols:Ml; Tpo; Mgdf; Mpllg��
NCBI Protein Information:thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
UniProt Protein Name:Thrombopoietin
UniProt Synonym Protein Names:C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Protein Family:Thyroid peroxidase
UniProt Gene Name:Thpo��
UniProt Entry Name:TPO_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P40226
Research Area:
Cytokines
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