Enzymes Recombinant Proteins

Mouse TGM2 Recombinant Protein (RPPB2315)

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RPPB2315

Description

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Product Name:Mouse TGM2 Recombinant Protein
Product Code:RPPB2315
Size:20µg
Species:Mouse
Target:TGM2
Synonyms:Protein-glutamine gamma-glutamyltransferase 2, G[a]h,TG2, TGase2, tTG, tTGas, Protein-glutamine gamma-glutamyltransferase 2, Tissuetransglutaminase, Transglutaminase C.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:TGM2 protein solution (0.5mg/ml) in Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHHSSGLVPRGSH MGSMAEELLLERCDLEIQAN GRDHHTADLC QEKLVLRRGQ RFRLTLYFEG RGYEASVDSL TFGAVTGPDPSEEAGTKARFSLSDNVEEGS WSASVLDQQD NVLSLQLCTP ANAPIGLYRL SLEASTGYQG SSFVLGHFIL LYNAWCPADDVYLDSEEERR EYVLTQQGFI YQGSVKFIKS VPWNFGQFED GILDTCLMLL DMNPKFLKNR SRDCSRRSSPIYVGRVVSAM VNCNDDQGVL LGRWDNNYGD GISPMAWIGS VDILRRWKEH GCQQVKYGQC WVFAAVACTVLRCLGIPTRV VTNYNSAHDQ NSNLLIEYFR NEFGELESNK SEMIWNFHCW VESWMTRPDL QPGYEGWQAIDPTPQEKSEG TYCCGPVSVR AIKEGDLSTK YDAPFVFAEV NADVVDWIRQ EDGSVLKSIN RSLVVGQKIS TKSVGRDDREDITHTYKYPE GSPEEREVFT KANHLNKLAE KEETGVAMRI RVGDSMSMGN DFDVFAHIGN DTSETRECRLLLCARTVSYN GVLGPECGTE DINLTLDPYS ENSIPLRILY EKYSGCLTES NLIKVRGLLI EPAANSYLLAERDLYLENPE IKIRVLGEPK QNRKLVAEVS LKNPLSDPLY DCIFTVEGAG LTKEQKSVEV SDPVPAGDLVKARVDLFPTD IGLHKLVVNF QCDKLKSVKG YRNVIIGPA

Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig.Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.

TGM2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 709 amino acids (1-686 a.a) and having a molecular mass of 79.4kDa.TGM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis.. Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:EC 2.3.2.13; Transferase

Cellular Component: cytoplasm; cytosol; extracellular matrix; focal adhesion; intrinsic to plasma membrane; membrane; mitochondrion; plasma membrane; proteinaceous extracellular matrix

Molecular Function:GTP binding; protein domain specific binding; protein-glutamine gamma-glutamyltransferase activity; transaminase activity

Biological Process: apoptotic cell clearance; blood vessel remodeling; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); elevation of mitochondrial calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; peptide cross-linking; positive regulation of apoptosis; positive regulation of cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of inflammatory response; positive regulation of smooth muscle cell proliferation; protein homooligomerization; reduction of endoplasmic reticulum calcium ion concentration

UniProt Code:P21981
NCBI GenInfo Identifier:6678329
NCBI Gene ID:21817
NCBI Accession:NP_033399.1
UniProt Related Accession:P21981
Molecular Weight:
NCBI Full Name:protein-glutamine gamma-glutamyltransferase 2
NCBI Synonym Full Names:transglutaminase 2, C polypeptide
NCBI Official Symbol:Tgm2��
NCBI Official Synonym Symbols:TG2; tTG; G[a]h; tTGas; TGase2��
NCBI Protein Information:protein-glutamine gamma-glutamyltransferase 2
UniProt Protein Name:Protein-glutamine gamma-glutamyltransferase 2
UniProt Synonym Protein Names:Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase-2; TGase-2
Protein Family:Protein-glutamine gamma-glutamyltransferase
UniProt Gene Name:Tgm2��
UniProt Entry Name:TGM2_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P21981
Research Area:
Enzymes
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