Enzymes Recombinant Proteins

Mouse TEK Recombinant Protein (RPPB2659)

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SKU:
RPPB2659
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q02858
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse TEK Recombinant Protein
Product Code:RPPB2659
Size:10µg
Species:Mouse
Target:TEK
Synonyms:Angiopoietin-1 receptor, Endothelial tyrosine kinase, HYK, STK1, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, mTIE2, p140 TEK, CD202b.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:TEK protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:AMDLILINSL PLVSDAETSL TCIASGWHPH EPITIGRDFE ALMNQHQDPLEVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGQAIR IRTMKMRQQA SFLPATLTMT VDRGDNVNISFKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA FTRLIVRRCEAQKWGPDCSR PCTTCKNNGV CHEDTGECIC PPGFMGRTCE KACEPHTFGR TCKERCSGPE GCKSYVFCLPDPYGCSCATG WRGLQCNEAC PSGYYGPDCK LRCHCTNEEI CDRFQGCLCS QGWQGLQCEK EGRPRMTPQIEDLPDHIEVN SGKFNPICKA SGWPLPTSEE MTLVKPDGTV LQPNDFNYTD RFSVAIFTVN RVLPPDSGVWVCSVNTVAGM VEKPFNISVK VLPEPLHAPN VIDTGHNFAI INISSEPYFG DGPIKSKKLF YKPVNQAWKYIEVTNEIFTL NYLEPRTDYE LCVQLARPGE GGEGHPGPVR RFTTASIGLP PPRGLSLLPK SQTALNLTWQ PIFTNSEDEFYVEVERRSLQ TTSDQQNIKV PGNLTSVLLS NLVPREQYTV RARVNTKAQG EWSEELRAWT LSDILPPQPENIKISNITDS TAMVSWTIVD GYSISSIIIR YKVQGKNEDQ HIDVKIKNAT VTQYQLKGLE PETTYHVDIFAENNIGSSNP AFSHELRTLP HSPASADLGG GKMLHHHHHH

TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1122 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 726 residue extracellular domain and a 353 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects.

TEK produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 730 amino acids (23-746 a.a.) andhaving a molecular mass of 81.6kDa (Migrates at 70-100kDa on SDS-PAGE underreducing conditions).TEK is expressed with a 6 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
UniProt Protein Details:

Protein type:Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Tie family

Cellular Component: microvillus; cell surface; integral to plasma membrane; basolateral plasma membrane; extracellular region; integral to membrane; actin filament; intercellular junction; lipid raft; cytoskeleton; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; basal plasma membrane; stress fiber; plasma membrane; cell junction

Molecular Function:transferase activity; protein binding; protein-tyrosine kinase activity; growth factor binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of cell adhesion; peptidyl-tyrosine phosphorylation; heart development; protein amino acid autophosphorylation; cell-matrix adhesion; positive regulation of cytokine secretion during immune response; protein amino acid phosphorylation; regulation of cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; cell-cell adhesion; positive regulation of focal adhesion formation; hemopoiesis; angiogenesis; vasculogenesis; Tie receptor signaling pathway; regulation of endothelial cell proliferation; response to retinoic acid; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; regulation of angiogenesis; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; patterning of blood vessels; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

UniProt Code:Q02858
NCBI GenInfo Identifier:730947
NCBI Gene ID:21687
NCBI Accession:Q02858.2
UniProt Related Accession:Q02858
Molecular Weight:125,701 Da
NCBI Full Name:Angiopoietin-1 receptor
NCBI Synonym Full Names:endothelial-specific receptor tyrosine kinase
NCBI Official Symbol:Tek��
NCBI Official Synonym Symbols:Hyk; STK1; Tie2; Tie-2; Cd202b; AA517024��
NCBI Protein Information:angiopoietin-1 receptor; p140 TEK; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2
UniProt Protein Name:Angiopoietin-1 receptor
UniProt Synonym Protein Names:Endothelial tyrosine kinase; HYK; STK1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; mTIE2; p140 TEK; CD_antigen: CD202b
Protein Family:Angiopoietin-1 receptor
UniProt Gene Name:Tek��
UniProt Entry Name:TIE2_MOUSE
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