Chemokines Recombinant Proteins

Mouse TECK Recombinant Protein (RPPB1266)

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SKU:
RPPB1266
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
O35903
Research Area:
Chemokines

Description

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Product Name:Mouse TECK Recombinant Protein
Product Code:RPPB1266
Size:20µg
Species:Mouse
Target:TECK
Synonyms:C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Filtered (0.2�m) and lyophilized from a concentrated (1mg/ml) solution in in 1�PBS, pH7.4.
Solubility:It is recommended to reconstitute the lyophilized TECK in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TECK should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSASDS QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N
Biological Activity:Determined by its ability to chemoattract human CCR9 transfected BaF3 mouse pro-B cells using a concentration range of 0.1-0.5 ug/ml.

CCL25 (Teck) is a novel CC chemokine, which is distantly related (about 20% amino acid sequence identity) to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development. Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.

TECK Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 14.1kDa. The TECK is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function:protein binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; positive regulation of cell-matrix adhesion; chemotaxis; leukocyte migration

UniProt Code:O35903
NCBI GenInfo Identifier:3122923
NCBI Gene ID:20300
NCBI Accession:O35903.1
Molecular Weight:16,714 Da
NCBI Full Name:C-C motif chemokine 25
UniProt Protein Name:C-C motif chemokine 25
UniProt Synonym Protein Names:Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine
Protein Family:C-C motif chemokine
UniProt Gene Name:Ccl25��
UniProt Entry Name:CCL25_MOUSE
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