Mouse ShhN PharmaGenie ELISA Kit (SBRS1526)
- SKU:
- SBRS1526
- Product Type:
- ELISA Kit
- Reactivity:
- Mouse
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
system_update_altDatasheet
Mouse ShhN PharmaGenie ELISA Kit (SBRS1526)
| Product SKU: | SBRS1526 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-9, IL-10, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA- 3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Uniprot: | Q62226 |
| Gene ID: | 20423 |
| Gene Names: | HHG1 / SHH |
| Synonyms: | Sonic hedgehog protein (SHH) (HHG-1) [Cleaved into: Sonic hedgehog protein N-product (Sonic hedgehog protein 19 kDa product) Sonic hedgehog protein C-product (Sonic hedgehog protein 27 kDa product)] |
| Target Species: | Mouse |
| Compatible Sample Types: | Cell and Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 5 pg/ml - 1000 pg/ml |
| Sensitivity: | 5 pg/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Mouse ShhN PharmaGenie ELISA Kit (SBRS1526) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
 | Standard Curves for Mouse ShhN PharmaGenie ELISA Kit (SBES1526) |
Related Products
Mouse ShhN PharmaGenie ELISA Kit (SBRS1525)
system_update_altDatasheetMouse ShhN PharmaGenie ELISA Kit (SBRS1525)Overview
Mouse Secretin PharmaGenie ELISA Kit (SBRS0080)
Mouse Secretin PharmaGenie ELISA Kit (SBRS0080)OverviewProduct SKU:SBRS0080Size:96TApplication:This ELISA kit is designed to detect human, mouse, and rat
Mouse Progesterone PharmaGenie ELISA Kit (SBRS0071)
OverviewProduct SKU:SBRS0071Size:96TApplication:This ELISA kit is designed to detect human, mouse, and rat Progesterone.Target Species:H
Mouse Urocortin PharmaGenie ELISA Kit (SBRS0085)
OverviewProduct SKU:SBRS0085Size:96TApplication:This ELISA kit is designed to detect human, mouse, and rat active Urocortin.Uniprot: