CD Antigens Recombinant Proteins

Mouse sCD40L Recombinant Protein (RPPB0896)

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SKU:
RPPB0896
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P27548
Research Area:
CD Antigens

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse sCD40L Recombinant Protein
Product Code:RPPB0896
Size:5µg
Species:Mouse
Target:sCD40L
Synonyms:CD40 ligand, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40lg, CD154, CD40-L, Cd40l, gp39, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, Tnfsf5.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:sCD40 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLTVKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQAGASVFVNVT EASQVIHRVG FSSFGLLKLH HHHHH

CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines . CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma , TNF-alpha , and IL2.

sCD40 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 155 amino acids (112-260 a.a.) and having a molecular mass of 17.2kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions).sCD40 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; cell; integral to membrane; extracellular region; plasma membrane; external side of plasma membrane

Molecular Function:CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; positive regulation of interleukin-12 production; isotype switching; positive regulation of immune response to tumor cell; signal transduction; positive regulation of interleukin-10 production; positive regulation of interferon-gamma production; positive regulation of MHC class II biosynthetic process; B cell differentiation; immunoglobulin secretion; positive regulation of B cell proliferation; immune response; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

UniProt Code:P27548
NCBI GenInfo Identifier:18314332
NCBI Gene ID:21947
NCBI Accession:P27548.2
UniProt Related Accession:P27548
Molecular Weight:29,370 Da
NCBI Full Name:CD40 ligand
NCBI Synonym Full Names:CD40 ligand
NCBI Official Symbol:Cd40lg��
NCBI Official Synonym Symbols:IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5��
NCBI Protein Information:CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5
UniProt Protein Name:CD40 ligand
UniProt Synonym Protein Names:T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
UniProt Gene Name:Cd40lg��
UniProt Entry Name:CD40L_MOUSE
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