Chemokines Recombinant Proteins

Mouse Rantes Recombinant Protein (RPPB1243)

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RPPB1243

Description

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Product Name:Mouse Rantes Recombinant Protein
Product Code:RPPB1243
Size:20µg
Species:Mouse
Target:Rantes
Synonyms:Small inducible cytokine A5, CCL5, T-cell-specific RANTES protein, SIS-delta, T cell- specific protein P228, TCP228, chemokine (C-C motif) ligand 5, SISd, SCYA5, RANTES, D17S136E, MGC17164.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Filtered (0.2�m) and lyophilized from a solution in 2�PBS, pH7.4.
Solubility:It is recommended to reconstitute the lyophilized Rantes in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Mouse Rantes although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse CCL5 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS
Biological Activity:The activity is determined by the ability to chemoattract total human lymphocytes population at a concentration between 1-10ng/ml.

Regulated upon Activation, Normal T-cell Expressed, and Secreted or RANTES is an 8 kDa protein classified as a chemotactic cytokine or chemokine. It has recently been renamed CCL5. RANTES is chemotactic for T cells, eosinophils and basophils and plays an active role in recruiting leukocytes into inflammatory sites.

Rantes Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7.8kDa. The Mouse Rantes is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Chemokine; Cell adhesionCellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellularMolecular Function: heparin binding; CCR1 chemokine receptor binding; protein homodimerization activity; protein self-association; chemokine activity; chemokine receptor binding; chemokine receptor antagonist activity; cytokine activity; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; CCR5 chemokine receptor binding; phospholipase activator activity; protein kinase activity; chemoattractant activityBiological Process: exocytosis; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; response to toxin; positive regulation of smooth muscle cell proliferation; chemotaxis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of homotypic cell-cell adhesion; pseudopodium formation; leukocyte adhesion; cell-cell signaling; positive chemotaxis; calcium ion transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; positive regulation of T cell proliferation; inflammatory response; lymphocyte chemotaxis; protein tetramerization; phospholipase D activation; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; MAPKKK cascade; positive regulation of cellular biosynthetic process; cellular calcium ion homeostasis; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cellular protein complex assembly; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of viral genome replication; response to cytokine stimulus; eosinophil chemotaxis; positive regulation of fever; immune response; positive regulation of neuron differentiation; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; regulation of insulin secretion; positive regulation of epithelial cell proliferation; positive regulation of phosphorylation; positive regulation of cell migration; regulation of T cell activation
UniProt Protein Details:
NCBI Summary:
UniProt Code:P30882
NCBI GenInfo Identifier:548926
NCBI Gene ID:20304
NCBI Accession:P30882.2
UniProt Secondary Accession:P30882
UniProt Related Accession:P30882
Molecular Weight:10,071 Da
NCBI Full Name:C-C motif chemokine 5
NCBI Synonym Full Names:chemokine (C-C motif) ligand 5
NCBI Official Symbol:Ccl5��
NCBI Official Synonym Symbols:SISd; Scya5; RANTES; TCP228; MuRantes��
NCBI Protein Information:C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES
UniProt Protein Name:C-C motif chemokine 5
UniProt Synonym Protein Names:MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES
Protein Family:C-C motif chemokine
UniProt Gene Name:Ccl5��
UniProt Entry Name:CCL5_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P30882
Research Area:
Chemokines
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