Growth Factors & Cytokines Recombinant Proteins
Mouse R-Spondin 1/RSPO1 Recombinant Protein (RPES3108)
- SKU:
- RPES3108
Description
Mouse R-Spondin 1/RSPO1 Recombinant Protein
RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
| Product Name: | Mouse R-Spondin 1/RSPO1 Recombinant Protein (RPES3108) |
| Product Code: | RPES3108 |
| Size: | 20µg |
| Species: | Mouse |
| Expressed Host: | CHO Stable Cells |
| Synonyms: | R-spondin,Rspondin |
| Accession: | NP_619624.2 |
| Sequence: | Met 1-Gln 265 |
| Fusion tag: | C-His |
| Activity: | Measured by its ability to induce activation of ?-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus. |
| Purity: | > 95 % as determined by SDS-PAGE |
| Mol Mass: | 28.5 kDa |
| AP Mol Mass: | 44 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Mouse |
Research Area: |
Growth Factors & Cytokines |
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Growth Factors & Cytokines Recombinant Proteins
