Enzymes Recombinant Proteins

Mouse PGK1 Recombinant Protein (RPPB2603)

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RPPB2603

Description

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Product Name:Mouse PGK1 Recombinant Protein
Product Code:RPPB2603
Size:25µg
Species:Mouse
Target:PGK1
Synonyms:Phosphoglycerate kinase 1 (EC:2.7.2.3), Pgk1, Pgk-1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:PGK1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V
Biological Activity:Specific activity: > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37�C.

PGK1 is an X-linked enzyme that has a major role in the glycolytic pathway. PGK1 is a glycolytic enzyme which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating an ATP molecule. PGK1 may also act as a cofactor for polymerase alpha. Defects in the PGK1 gene are usually associated with chronic hemolytic anemia, though it can be accompanied by either mental retardation or muscular disease (rhabdomyolysis). Overexpression of PGK1 and its signalling targets are possibly an expression-pathway in diffuse primary gastric carcinomas promoting peritoneal dissemination. It was shown that PGK1 is differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.

PGK1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441 amino acids (1-417 a.a) and having a molecular mass of 47.1kDa.PGK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein).Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.2.3; Kinase, otherChromosomal Location of Human Ortholog: X D|X 47.36 cMCellular Component: cytosol; lipid raft; membraneMolecular Function: ADP binding; ATP binding; phosphoglycerate kinase activityBiological Process: carbohydrate metabolic process; gluconeogenesis; glycolysis
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
UniProt Code:P09411
NCBI GenInfo Identifier:70778976
NCBI Gene ID:18655
NCBI Accession:NP_032854.2
UniProt Secondary Accession:P09411,Q3TPE6, Q3UKV8, Q5XJE7
UniProt Related Accession:P09411
Molecular Weight:44,550 Da
NCBI Full Name:phosphoglycerate kinase 1
NCBI Synonym Full Names:phosphoglycerate kinase 1
NCBI Official Symbol:Pgk1��
NCBI Official Synonym Symbols:Pgk-1��
NCBI Protein Information:phosphoglycerate kinase 1
UniProt Protein Name:Phosphoglycerate kinase 1
UniProt Synonym Protein Names:
Protein Family:
UniProt Gene Name:Pgk1��
UniProt Entry Name:
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P09411
Research Area:
Enzymes
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