Growth Factors & Cytokines Recombinant Proteins
Mouse PDGF BB Recombinant Protein (RPPB0840)
- SKU:
- RPPB0840
Description
| Product Name: | Mouse PDGF BB Recombinant Protein |
| Product Code: | RPPB0840 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | PDGF BB |
| Synonyms: | Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from 10mM NaCitrate pH-3.0. |
| Solubility: | It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT |
| Biological Activity: | The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml. |
PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
Platelet-Derived Growth Factor BB Mouse Recombinant produced in E.coli, is a homodimeric, non-glycosylated, polypeptide chain containing 2x110 (total of 2 chains 220aa) amino acids and having a total molecular weight of 24.7 kDa.PDGF-BB is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Oncoprotein Cellular Component: extracellular space; cell surface; membrane; basolateral plasma membrane; cytoplasm; extracellular region Molecular Function:collagen binding; identical protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity; receptor binding Biological Process: embryonic placenta development; peptidyl-tyrosine phosphorylation; positive regulation of cyclin-dependent protein kinase activity; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; positive regulation of smooth muscle cell proliferation; platelet-derived growth factor receptor signaling pathway; positive regulation of fibroblast growth factor receptor signaling pathway; cell projection biogenesis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; positive chemotaxis; positive regulation of MAPKKK cascade; negative regulation of vasodilation; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; positive regulation of mitotic cell cycle, embryonic; regulation of peptidyl-tyrosine phosphorylation; negative regulation of cell migration; substrate-bound cell migration; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of cell division; activation of protein kinase activity; positive regulation of vasoconstriction; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; blood coagulation; negative regulation of transcription, DNA-dependent; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication; positive regulation of cell migration |
| UniProt Code: | P31240 |
| NCBI GenInfo Identifier: | 400744 |
| NCBI Gene ID: | 18591 |
| NCBI Accession: | P31240.1 |
| UniProt Secondary Accession: | P31240,Q6P3C4, |
| UniProt Related Accession: | P31240 |
| Molecular Weight: | 27,382 Da |
| NCBI Full Name: | Platelet-derived growth factor subunit B |
| NCBI Synonym Full Names: | platelet derived growth factor, B polypeptide |
| NCBI Official Symbol: | Pdgfb�� |
| NCBI Official Synonym Symbols: | Sis; PDGF-B�� |
| NCBI Protein Information: | platelet-derived growth factor subunit B; c-sis; PDGF-2; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide |
| UniProt Protein Name: | Platelet-derived growth factor subunit B |
| UniProt Synonym Protein Names: | PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis |
| UniProt Gene Name: | Pdgfb�� |
| UniProt Entry Name: | PDGFB_MOUSE |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Mouse |
Uniprot: |
P31240 |
Research Area: |
Growth Factors & Cytokines |
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