Growth Factors & Cytokines Recombinant Proteins

Mouse PDGF AA Recombinant Protein (RPPB0834)

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SKU:
RPPB0834
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P20033
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse PDGF AA Recombinant Protein
Product Code:RPPB0834
Size:10µg
Species:Mouse
Target:PDGF AA
Synonyms:Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized with no additives.
Solubility:It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18M?-cm H2O at a concentration ranging between 0.1-0.5mg per 1ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
Biological Activity:Established by the dose-dependent stimulation of Balb/c 3T3 cells proliferation. The expected ED50� for this effect is 8-10 ng/ml corresponding to a specific activity of 100,000-125,000IU/mg.

PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.

PDGF-AA Mouse Recombinant is a disulfide linked homodimeric, non-glycosylated, polypeptide chain containing 2 x 126 amino acids and having a total molecular mass of 28.9 kDa. PDGF-AA is purified by proprietary chromatographic techniques.

UniProt Protein Function:PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Cellular Component: extracellular space; microvillus; cell surface; membrane; extracellular region

Molecular Function:collagen binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; multicellular organismal development; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; positive regulation of MAPKKK cascade; hair follicle development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; organ morphogenesis; gut development; positive regulation of cell division; blood coagulation; actin cytoskeleton organization and biogenesis; alveolus development; positive regulation of DNA replication; positive regulation of cell migration

UniProt Code:P20033
NCBI GenInfo Identifier:1709615
NCBI Gene ID:18590
NCBI Accession:P20033.2
UniProt Related Accession:P20033
Molecular Weight:22,267 Da
NCBI Full Name:Platelet-derived growth factor subunit A
NCBI Synonym Full Names:platelet derived growth factor, alpha
NCBI Official Symbol:Pdgfa��
NCBI Protein Information:platelet-derived growth factor subunit A; PDGF-1; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha polypeptide
UniProt Protein Name:Platelet-derived growth factor subunit A
UniProt Synonym Protein Names:PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide
UniProt Gene Name:Pdgfa��
UniProt Entry Name:PDGFA_MOUSE
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