Mouse Obestatin PharmaGenie ELISA Kit (SBRS0068)

1. Pre-Coated 96-well Strip Microplate
2. Wash Buffer
3. Standard Peptide
4. Assay Diluent(s)
5. Biotinylated Peptide
6. HRP-Streptavidin
7. TMB One-Step Substrate
8. Stop Solution
9. Assay Diagram
10. Positive Control Sample
11. Capture Antibody
12. Technical Manual

Other materials and equipment required:

The Assay Genie Mouse Obestatin PharmaGenie ELISA Kit (SBRS0068) will require other equipment and materials to carry out the assay. Please see list below for further details.

• Distilled or deionized water
• Precision pipettes to deliver 2 ul to 1 ml volumes
• Adjustable 1-25 ml pipettes for reagent preparation
• 100 ml and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

1. Pre-Coated 96-well Strip Microplate
2. Wash Buffer
3. Standard Peptide
4. Assay Diluent(s)
5. Biotinylated Peptide
6. HRP-Streptavidin
7. TMB One-Step Substrate
8. Stop Solution
9. Assay Diagram
10. Positive Control Sample
11. Capture Antibody
12. Technical Manual

Other materials and equipment required:

The Assay Genie Mouse Obestatin ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

• Distilled or deionized water
• Precision pipettes to deliver 2 ul to 1 ml volumes
• Adjustable 1-25 ml pipettes for reagent preparation
• 100 ml and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Obestatin is a 24-aa peptide hormone that is produced in the stomach and small intestine of humans and several other mammals. It is encoded by the same gene that also encodes adipokine Ghrelin, which is initially expressed as a preprohormone that is subsequently cleaved into two smaller peptides, Ghrelin and Obestatin. A model has been proposed suggesting a "Yin-Yang" relationship between the two peptides, that Ghrelin and Obestatin have countering effects to each other. There are reports using Ghrelin knockout mice studies which showed that removing the Ghrelin gene from mice did not significantly reduce their appetite. Additional reports show that Obestatin antagonizes growth hormone secretion and food intake induced by Ghrelin. Obestatin has shown some potential clinical applications. Studies on the Obestatin/Ghrelin ratio in the gastrointestinal tract and plasma are associated with metabolic diseases such as irritable bowel syndrome, obesity and type II diabetes mellitus.