Mouse Immunology ELISA Kits

Mouse NT proBNP ELISA Kit

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SKU:
MOFI01007
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
NT-proBNP, N-Terminal Pro-Brain Natriuretic Peptide
Reactivity:
Mouse

Description

Mouse NT proBNP ELISA Kit

The Mouse NT-proBNP ELISA Kit is specifically designed for the quantitative measurement of N-terminal pro-brain natriuretic peptide (NT-proBNP) levels in mouse serum, plasma, and tissue lysates. NT-proBNP is a valuable biomarker for various cardiac conditions, particularly heart failure, in mice. This kit allows researchers to accurately assess NT-proBNP levels, aiding in the diagnosis, prognosis, and monitoring of cardiac diseases in mouse models.

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Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse NT proBNP ELISA Kit

Product Code:

MOFI01007

Size:

96 Assays

Alias:

NT-proBNP, N-Terminal Pro-Brain Natriuretic Peptide

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse NT-proBNP concentrations in serum plasma and other biological fluids.

Sensitivity:

9.375pg/ml

Range:

15.625-1000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse NT-proBNP and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse NT-proBNP in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

90-102

98

EDTA plasma (n=5)

85-105

92

UFH plasma (n=5)

87-102

95

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse NT-proBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

91-102%

87-102%

90-106%

EDTA plasma (n=5)

83-101%

88-98%

86-100%

UFH Plasma (n=5)

85-94%

80-100%

82-97%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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