Mouse Neuro Discovery Array (23 targets) (SARB0091)

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SKU:
SARB0091

Description

system_update_altDatasheet
Product Sku:SARB0091
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:23
Gene Symbols:AGER, CCL17, CCL2, CCL3, CSF1, CSF3, CX3CL1, CXCL1, CXCL12, CXCL5|FASLG, IFNG, IGF1, IL10, IL1A, IL1B, IL4, IL6, MMP2, MMP3, TGFB1, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Fas Ligand (TNFSF6)
  • Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6
  • Gene ID: 14103
  • Accession #: P41047
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1 FKN NTT SCYD1 A-152E5.2
  • Gene ID: 14825
  • Accession #: O35188
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 12985
  • Accession #: P09920
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 15978
  • Accession #: P01580
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 16000
  • Accession #: P05017
IL-10
  • Gene Symbol: IL10
  • Gene ID: 16153
  • Accession #: P18893
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 16175
  • Accession #: P01582
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 16176
  • Accession #: P10749
IL-4
  • Gene Symbol: IL4
  • Gene ID: 16189
  • Accession #: P07750
IL-6
  • Gene Symbol: IL6
  • Gene ID: 16193
  • Accession #: P08505
KC (CXCL1)
  • Gene Symbol: Cxcl1 Gro Gro1 Mgsa Scyb1
  • Gene ID: 14825
  • Accession #: P12850
LIX
  • Gene Symbol: Cxcl5 Scyb5
  • Gene ID: 20311
  • Accession #: P50228
MCP-1 (CCL2)
  • Gene Symbol: CCL2 SCYA2
  • Gene ID: 20296
  • Accession #: P10148
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 12977
  • Accession #: P07141
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 20302
  • Accession #: P10855
MMP-2
  • Gene Symbol: MMP2 CLG4A
  • Gene ID: 17390
  • Accession #: P33434
MMP-3
  • Gene Symbol: MMP3 STMY1
  • Gene ID: 17392
  • Accession #: P28862
RAGE
  • Gene Symbol: N/A
  • Gene ID: 26448
  • Accession #: Q9WVS4
TARC (CCL17)
  • Gene Symbol: CCL17 SCYA17 TARC
  • Gene ID: 20295
  • Accession #: Q9WUZ6
SDF-1 alpha (CXCL12 alpha)
  • Gene Symbol: CXCL12 SDF1 SDF1A SDF1B
  • Gene ID: 20315
  • Accession #: P40224
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 21803
  • Accession #: P04202
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 21926
  • Accession #: P06804
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 22339
  • Accession #: Q00731
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Neuro Discovery C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Fas Ligand (TNFSF6)
Analytes:
Fractalkine (CX3CL1)
Analytes:
GCSF
Analytes:
IFN-gamma
Analytes:
IGF-1
Analytes:
IL-10
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-4
Analytes:
IL-6
Analytes:
KC (CXCL1)
Analytes:
LIX
Analytes:
MCP-1 (CCL2)
Analytes:
M-CSF
Analytes:
MIP-1 alpha (CCL3)
Analytes:
MMP-2
Analytes:
MMP-3
Analytes:
RAGE
Analytes:
TARC (CCL17)
Analytes:
SDF-1 alpha (CXCL12 alpha)
Analytes:
TGF beta 1
Analytes:
TNF alpha
Analytes:
VEGF-A
Reactivity:
Mouse
Applications:
Multiplex Array
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