Mouse MMP-10 PharmaGenie ELISA Kit (SBRS1480)
- SKU:
- SBRS1480
- Product Type:
- ELISA Kit
- Reactivity:
- Mouse
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
system_update_altDatasheet
Mouse MMP-10 PharmaGenie ELISA Kit (SBRS1480)
| Product SKU: | SBRS1480 |
| Size: | 96T |
| Application: | This ELISA antibody pair recognizes Mouse MMP-10. |
| Uniprot: | O55123 |
| Gene ID: | 17384 |
| Gene Names: | MMP10 |
| Synonyms: | Stromelysin-2 (SL-2) (EC 3.4.24.22) (Matrix metalloproteinase-10) (MMP-10) (Transin-2) |
| Target Species: | Mouse |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 2 pg/ml - 1000 pg/ml |
| Sensitivity: | 2 pg/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | May be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Mouse MMP-10 PharmaGenie ELISA Kit (SBRS1480) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
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