Chemokines Recombinant Proteins

Mouse MIP 3b Recombinant Protein (RPPB1224)

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SKU:
RPPB1224
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
O70460
Research Area:
Chemokines

Description

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Product Name:Mouse MIP 3b Recombinant Protein
Product Code:RPPB1224
Size:20µg
Species:Mouse
Target:MIP 3b
Synonyms:Small inducible cytokine A19, CCL19, Macrophage inflammatory protein 3 beta, MIP-3- beta, EBI1-ligand chemokine, ELC, Beta chemokine exodus-3, CK beta-11, chemokine (C-C motif) ligand 19, CKb11, MIP3B, MIP-3b, SCYA19, MGC34433, Epstein-Barr virus-induced molecule 1 ligand chemokine, EBI1-ligand chemokine.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The CCL19 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility:It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL19 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala
Biological Activity:The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration range of 8-80 ng/ml.

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.

Macrophage Inflammatory Protein-3 beta Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. The MIP-3b is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: cell; intracellular; cytosol; external side of plasma membrane

Molecular Function:CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding

Biological Process: positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; positive regulation of phosphoinositide 3-kinase activity; dendritic cell chemotaxis; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; establishment of T cell polarity

UniProt Code:O70460
NCBI GenInfo Identifier:6094383
NCBI Gene ID:100039053
NCBI Accession:O70460.1
Molecular Weight:11,911 Da
NCBI Full Name:C-C motif chemokine 19
NCBI Synonym Full Names:predicted gene 2023
NCBI Official Symbol:Gm2023��
NCBI Official Synonym Symbols:ENSMUSG00000078736; ENSMUSG00000078738��
NCBI Protein Information:predicted gene 2023
UniProt Protein Name:C-C motif chemokine 19
UniProt Synonym Protein Names:Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Small-inducible cytokine A19
UniProt Gene Name:Ccl19��
UniProt Entry Name:CCL19_MOUSE
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