Chemokines Recombinant Proteins

Mouse MIP 1a Recombinant Protein (RPPB1214)

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SKU:
RPPB1214
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P10855
Research Area:
Chemokines

Description

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Product Name:Mouse MIP 1a Recombinant Protein
Product Code:RPPB1214
Size:10µg
Species:Mouse
Target:MIP 1a
Synonyms:Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA, TY-5, SIS-alpha, L2G25B.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from 1mg/ml solution containing no additives.
Solubility:It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 99.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Tyr-Gly-Ala
Biological Activity:The Activity is calculated by the ability to chemoattract of Balb3/C splenocytes using 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-a from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.

Macrophage Inflammatory Protein-1 alpha Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7820 Dalton. The MIP-1a is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL3L1: Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1- infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function:protein binding; chemokine activity; cytokine activity

Biological Process: neutrophil chemotaxis; positive regulation of osteoclast differentiation; leukocyte chemotaxis; positive regulation of interleukin-1 beta secretion; positive regulation of tumor necrosis factor production; chemotaxis; macrophage chemotaxis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; astrocyte cell migration; positive regulation of inflammatory response

UniProt Code:P10855
NCBI GenInfo Identifier:127079
NCBI Gene ID:20302
NCBI Accession:P10855.2
UniProt Secondary Accession:P10855,P14096,
UniProt Related Accession:P10855
Molecular Weight:10,345 Da
NCBI Full Name:C-C motif chemokine 3
NCBI Synonym Full Names:chemokine (C-C motif) ligand 3
NCBI Official Symbol:Ccl3��
NCBI Official Synonym Symbols:Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha��
NCBI Protein Information:C-C motif chemokine 3; TY-5; L2G25B; MIP1 (a); SIS-alpha; MIP-1 alpha; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; heparin-binding chemotaxis protein; macrophage inflammatory protein-1alpha; macrophage inflammatory protein 1-alpha
UniProt Protein Name:C-C motif chemokine 3
UniProt Synonym Protein Names:Heparin-binding chemotaxis protein; L2G25B; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; SIS-alpha; Small-inducible cytokine A3; TY-5
Protein Family:C-C motif chemokine
UniProt Gene Name:Ccl3��
UniProt Entry Name:CCL3_MOUSE
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