Chemokines Recombinant Proteins

Mouse MCP 5 Recombinant Protein (RPPB1199)

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SKU:
RPPB1199
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q62401
Research Area:
Chemokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse MCP 5 Recombinant Protein
Product Code:RPPB1199
Size:20µg
Species:Mouse
Target:MCP 5
Synonyms:C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, MCP-5, Small-inducible cytokine A12, Ccl12, Mcp5, Scya12.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2�m filtered concentrated solution in 1�PBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized MCP5 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized CCL12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCP5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Biological Activity:Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.

MCP-5 (CCL12) is a cloned mouse CC chemokine most closely related to human MCP1 (66% amino acid sequence identity in the mature protein). MCP5 is expressed constitutively in the thymus and the lymph nodes. Under inflammatory conditions, moreover CCL12 expression is induced in activated macrophages and mast cells. The mouse MCP1 gene is mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, MCP-5 also chemoattracts eosinophils. CCL12 is a functional ligand for CCR2.

MCP-5 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 82 amino acids and having a molecular mass of 9.3kDa. The MCP5 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function:CCR2 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of leukocyte migration; MAPKKK cascade; cytoskeleton organization and biogenesis; chemotaxis; macrophage chemotaxis; monocyte chemotaxis; regulation of cell shape; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; immune response; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; astrocyte cell migration

UniProt Code:Q62401
NCBI GenInfo Identifier:2493663
NCBI Gene ID:102641905
NCBI Accession:Q62401.1
UniProt Secondary Accession:Q62401,Q9QYD6,
Molecular Weight:11,659 Da
NCBI Full Name:C-C motif chemokine 12
NCBI Official Symbol:LOC102641905��
NCBI Protein Information:c-C motif chemokine 12-like
UniProt Protein Name:C-C motif chemokine 12
UniProt Synonym Protein Names:MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12
Protein Family:C-C motif chemokine
UniProt Gene Name:Ccl12��
UniProt Entry Name:CCL12_MOUSE
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