Description
| Product Name: | Mouse MCP 2 Recombinant Protein |
| Product Code: | RPPB1192 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | MCP 2 |
| Synonyms: | Small inducible cytokine A8, CCL8, Monocyte chemotactic protein 2, MCP-2, Monocyte chemoattractant protein 2, HC14, chemokine (C-C motif) ligand 8, MCP2, SCYA8, SCYA10, AB023418, 1810063B20Rik. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized CCL8in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized MCP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL8 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP |
| Biological Activity: | Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg. |
Chemokine (C-C motif) ligand 8 (CCL8) is a small cytokine belonging to the CC chemokine family that was once called monocyte chemotactic protein-2 (MCP-2). The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates a many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B and CCR5.
Monocyte Chemotactic Protein-2 Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8507 Dalton. The MCP-2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family. |
| UniProt Protein Details: | Protein type:Chemokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis Cellular Component: extracellular space; extracellular region Molecular Function:heparin binding; chemokine activity; cytokine activity Biological Process: immune response; inflammatory response; chemotaxis; positive regulation of myoblast differentiation |
| UniProt Code: | Q9Z121 |
| NCBI GenInfo Identifier: | 13431906 |
| NCBI Gene ID: | 102641794 |
| NCBI Accession: | Q9Z121.1 |
| Molecular Weight: | 11,017 Da |
| NCBI Full Name: | C-C motif chemokine 8 |
| NCBI Official Symbol: | LOC102641794�� |
| NCBI Protein Information: | c-C motif chemokine 8-like |
| UniProt Protein Name: | C-C motif chemokine 8 |
| UniProt Synonym Protein Names: | Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8 |
| Protein Family: | C-C motif chemokine |
| UniProt Gene Name: | Ccl8�� |
| UniProt Entry Name: | CCL8_MOUSE |
