Growth Factors & Cytokines Recombinant Proteins
Mouse M CSF Recombinant Protein (RPPB0753)
- SKU:
- RPPB0753
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P07141
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Mouse M CSF Recombinant Protein |
| Product Code: | RPPB0753 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | M CSF |
| Synonyms: | CSF-1, Lanimostim, MCSF, M-CSF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a sterile (0.2�m) filtered solution containing 10mM sodium phosphate, 50mM sodium chloride, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized M-CSF in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP |
| Biological Activity: | The ED50, as calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells is 1.33ng/ml corresponding to a specific activity of 7.5x105 units/mg. |
Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.MCSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cytokine Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; plasma membrane; receptor complex Molecular Function:protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; positive regulation of multicellular organism growth; osteoclast differentiation; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; regulation of ossification; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; positive regulation of cell migration |
| UniProt Code: | P07141 |
| NCBI GenInfo Identifier: | 1345843 |
| NCBI Gene ID: | 12977 |
| NCBI Accession: | P07141.2 |
| UniProt Secondary Accession: | P07141,Q3U395, Q8R3C8, |
| UniProt Related Accession: | P07141 |
| Molecular Weight: | 29,353 Da |
| NCBI Full Name: | Macrophage colony-stimulating factor 1 |
| NCBI Synonym Full Names: | colony stimulating factor 1 (macrophage) |
| NCBI Official Symbol: | Csf1�� |
| NCBI Official Synonym Symbols: | op; Csfm; MCSF; C87615�� |
| NCBI Protein Information: | macrophage colony-stimulating factor 1; osteopetrosis |
| UniProt Protein Name: | Macrophage colony-stimulating factor 1 |
| Protein Family: | C-C motif chemokine |
| UniProt Gene Name: | Csf1�� |
| UniProt Entry Name: | CSF1_MOUSE |
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