Description
| Product Name: | Mouse LGMN Recombinant Protein |
| Product Code: | RPPB1903 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | LGMN |
| Synonyms: | Legumain,Asparaginyl endopeptidase, Protease, cysteine 1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | LGMN protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQIIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKSGPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMMNHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVEDLTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTILKRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTAHDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAMDKVCLSHYLE HHHHHH |
Legumain, also known as LGMN is a lysosomal cysteine protease which is foundin all mouse tissues, however it was mainly abundant in the kidney as well asplacenta. LGMN plays an essential role in the endosomal/lysosomal degradationsystem as the Legumain deficiency causes the accumulation of pro cathepsins B, H& L, another group of lysosomal cysteine proteases. Furthermore, overexpression of LGMN in tumors is important for invasion/metastasis.
LGMN produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 426 amino acids (18-435a.a.) andhaving a molecular mass of 48.6kDa.�(Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).LGMN is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis. [provided by RefSeq, Aug 2016] |
| UniProt Code: | O89017 |
| NCBI GenInfo Identifier: | 7242187 |
| NCBI Gene ID: | 19141 |
| NCBI Accession: | NP_035305.1 |
| UniProt Secondary Accession: | O89017 |
| UniProt Related Accession: | O89017 |
| Molecular Weight: | 49,373 Da |
| NCBI Full Name: | legumain preproprotein |
| NCBI Synonym Full Names: | legumain |
| NCBI Official Symbol: | Lgmn�� |
| NCBI Official Synonym Symbols: | AEP; Prsc1; AI746452; AU022324�� |
| NCBI Protein Information: | legumain |
| UniProt Protein Name: | Legumain |
| UniProt Synonym Protein Names: | Asparaginyl endopeptidase; Protease, cysteine 1 |
| Protein Family: | |
| UniProt Gene Name: | Lgmn�� |
| UniProt Entry Name: |
