Growth Factors & Cytokines Recombinant Proteins
Mouse Leptin tA Recombinant Protein (RPPB0725)
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- SKU:
- RPPB0725
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Mouse Leptin tA Recombinant Protein |
| Product Code: | RPPB0725 |
| Size: | 50µg |
| Species: | Mouse |
| Target: | Leptin tA |
| Source: | Escherichia Coli |
| Physical Appearance: | White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100�g/ml, which can then be further diluted with other aqueous solutions. |
| Stability: | Lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 99.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln |
| Biological Activity: | ProSpec�s Leptin Antagonist Triple Mutant Mouse Recombinant is capable of inhibiting Leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays. |
Leptin Antagonist Triple Mutant Mouse Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, LEP was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Mouse Recombinant was purified by proprietary chromatographic techniques.
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