Enzymes Recombinant Proteins

Mouse LDHB Recombinant Protein (RPPB1901)

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SKU:
RPPB1901
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P16125
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse LDHB Recombinant Protein
Product Code:RPPB1901
Size:5µg
Species:Mouse
Target:LDHB
Synonyms:L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, LDH-H, Ldh-2, Ldh2, Ldhb.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:LDHB protein solution (1mg/ml) containing 20mM Tris-HCl (pH8.0), 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL
Biological Activity:Specific activity is > 150 units/mg, in which one unit will 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.

LDHB Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (1-334 a.a) and having a molecular mass of 39kDa.LDHB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
UniProt Code:P16125
NCBI GenInfo Identifier:126042
NCBI Gene ID:16832
NCBI Accession:P16125.2
UniProt Secondary Accession:P16125,Q545Y4
UniProt Related Accession:P16125
Molecular Weight:36,572 Da
NCBI Full Name:L-lactate dehydrogenase B chain
NCBI Synonym Full Names:lactate dehydrogenase B
NCBI Official Symbol:Ldhb��
NCBI Official Synonym Symbols:Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582��
NCBI Protein Information:L-lactate dehydrogenase B chain
UniProt Protein Name:L-lactate dehydrogenase B chain
UniProt Synonym Protein Names:LDH heart subunit; LDH-H
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:Ldhb��
UniProt Entry Name:
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system_update_altDatasheetHuman LDH-B/LDHB Recombinant Protein L-Lactate Dehydrogenase B Chain (LDH-B) is a member of the lactate dehydrogenase family that consists of three members, LDH-A, LDH-B