Growth Factors & Cytokines Recombinant Proteins

Mouse KGF Recombinant Protein (RPPB0696)

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SKU:
RPPB0696
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P36363
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse KGF Recombinant Protein
Product Code:RPPB0696
Size:10µg
Species:Mouse
Target:KGF
Synonyms:HBGF-7, FGF7, FGF-7, KGF, Keratinocyte growth factor, Fibroblast growth factor 7, Heparin-binding growth factor 7.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-8 and 0.1M NaCl.
Solubility:It is recommended to reconstitute the lyophilized KGF in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized KGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Amino Acid Sequence:MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT
Biological Activity:The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 100,000 Units/mg.

KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Keratinocyte Growth Factor-1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa.The FGF-7 is purified by proprietary chromatographic techniques.

UniProt Protein Function:FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Cytokine

Cellular Component: Golgi apparatus; extracellular space; cell; extracellular region

Molecular Function:heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; fibroblast growth factor receptor binding; chemoattractant activity; receptor binding

Biological Process: positive regulation of keratinocyte migration; hair follicle morphogenesis; fibroblast growth factor receptor signaling pathway; wound healing; mesenchymal cell proliferation; phospholipid biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; ovarian follicle development; positive regulation of cell division; actin cytoskeleton reorganization; positive regulation of cell proliferation; phosphatidylcholine biosynthetic process; surfactant homeostasis; positive regulation of epithelial cell proliferation; lung development

UniProt Code:P36363
NCBI GenInfo Identifier:6679785
NCBI Gene ID:14178
NCBI Accession:NP_032034.1
UniProt Related Accession:P36363,Q544I6,Q8C386
Molecular Weight:22,347 Da
NCBI Full Name:fibroblast growth factor 7
UniProt Protein Name:Fibroblast growth factor 7
UniProt Synonym Protein Names:Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor
UniProt Gene Name:Fgf7��
UniProt Entry Name:FGF7_MOUSE
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