Cytokines Recombinant Proteins
Mouse IL 21 Recombinant Protein (RPPB0634)
- SKU:
- RPPB0634
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q9ES17
- Research Area:
- Cytokines
Description
| Product Name: | Mouse IL 21 Recombinant Protein |
| Product Code: | RPPB0634 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL 21 |
| Synonyms: | Interleukin-21, IL-21, Il21. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Mouse IL-21 was lyophilized from 20mM NaHCO3, pH 8.5. |
| Solubility: | It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Ser-Ser |
| Biological Activity: | The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells. |
IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (�C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
Interleukin-21 Mouse Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Apoptosis; Cytokine; Cell development/differentiation; Secreted, signal peptide Cellular Component: extracellular space; extracellular region Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; immune response; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process |
| UniProt Code: | Q9ES17 |
| NCBI GenInfo Identifier: | 55976590 |
| NCBI Gene ID: | 60505 |
| NCBI Accession: | Q9ES17.1 |
| UniProt Related Accession: | Q9ES17 |
| Molecular Weight: | 16,811 Da |
| NCBI Full Name: | Interleukin-21 |
| NCBI Synonym Full Names: | interleukin 21 |
| NCBI Official Symbol: | Il21�� |
| NCBI Official Synonym Symbols: | IL-21�� |
| NCBI Protein Information: | interleukin-21 |
| UniProt Protein Name: | Interleukin-21 |
| UniProt Gene Name: | Il21�� |
| UniProt Entry Name: | IL21_MOUSE |
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