Mouse Immunology ELISA Kits

Mouse IL 18 ELISA Kit

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SKU:
MOFI00929
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P70380
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
IL-18, Interleukin 18, IL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor
Reactivity:
Mouse
Research Area:
Immunology

Description

Mouse IL 18 ELISA Kit

IL-18, also known as Interleukin-18, is a cytokine that plays a crucial role in the regulation of the immune system. It is involved in modulating the immune response and has significant implications in various disease processes. The Mouse IL-18 ELISA Kit is specifically designed for the quantitative measurement of Interleukin-18 (IL-18) levels in mouse samples and enables researchers to study IL-18 levels in biological samples, contributing to a better understanding of immune system regulation and disease pathogenesis.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse IL 18 ELISA Kit

Product Code:

MOFI00929

Size:

96 Assays

Alias:

IL-18, Interleukin 18, IL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-18 concentrations in serum plasma and other biological fluids.

Sensitivity:

9.375pg/ml

Range:

15.625-1000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse IL-18 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse IL-18 in samples.

1

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

89-105

97

EDTA plasma (n=5)

86-100

93

UFH plasma (n=5)

85-100

91

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

86-101%

86-104%

92-103%

EDTA plasma (n=5)

85-96%

84-90%

86-100%

UFH Plasma (n=5)

83-89%

85-96%

89-100%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

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Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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