Mouse Immunology ELISA Kits

Mouse IL-10 ELISA Kit

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SKU:
MOFI00054
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18893
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
IL-10, Interleukin 10, IL10, IL10A, CSIF, TGIF
Reactivity:
Mouse

Description

Mouse IL-10 ELISA Kit

Interleukin-10 (IL-10) is a vital immunoregulatory cytokine that plays a significant role in the immune system's response to infections and inflammatory processes. The Mouse IL-10 kit allows researchers to accurately determine the concentration of IL-10 in different samples, such as serum, plasma, cell culture supernatants, and tissue homogenates. This information is invaluable in studying the role of IL-10 in various physiological and pathological processes, including infectious diseases, autoimmune conditions, inflammatory disorders, cancer, and other immune-related disorders.

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Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse IL-10 ELISA Kit

Product Code:

MOFI00054

Size:

96 Assays

Alias:

IL-10, Interleukin 10, IL10, IL10A, CSIF, TGIF

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-10 concentrations in serum plasma and other biological fluids.

Sensitivity:

9.375pg/ml

Range:

15.625-1000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse Il-10 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse IL-10 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

85-101

94

EDTA plasma (n=5)

89-105

97

UFH plasma (n=5)

88-100

96

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

89-100%

86-104%

87-105%

EDTA plasma (n=5)

84-101%

84-93%

88-99%

UFH Plasma (n=5)

83-98%

80-94%

85-96%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

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Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C