Cytokines Recombinant Proteins

Mouse IHH Recombinant Protein (RPPB0442)

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SKU:
RPPB0442
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P97812
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse IHH Recombinant Protein
Product Code:RPPB0442
Size:25µg
Species:Mouse
Target:IHH
Synonyms:Indian hedgehog protein, IHH, HHG-2, BDA1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2�m filtered concentrated solution in 1�PBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized IHH in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IHH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Biological Activity:Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 3.0-10.0?g/ml corresponding to a specific activity of 100-334units/mg.

IHH belongs to the hedgehog family of secreted signaling molecules. Hedgehog proteins are vital regulators of various developmental processes including growth, patterning and morphogenesis. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). IHH has a specific role in bone growth and differentiation. In addition, IHH is involved in yolk sac vasculogenesis, having a central role in differentiation of epiblast cells into endothelial and red blood cells. IHH mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage in growth regions of the developing bone. IHH gene mutations cause the brachydactyly type A1 which is characterized by shortening or malformation of the phalanges and also the acrocapitofemoral dysplasia.

IHH Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8kDa. The IHH is purified by proprietary chromatographic techniques.

UniProt Protein Function:IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.Cellular Component: plasma membrane; proteinaceous extracellular matrixMolecular Function: calcium ion binding; patched binding; protein bindingBiological Process: bone resorption; camera-type eye development; cartilage development; cell differentiation; cell fate specification; cell maturation; embryonic camera-type eye morphogenesis; embryonic development; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic pattern specification; heart looping; in utero embryonic development; morphogenesis of a branching structure; multicellular organism growth; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of eye pigmentation; negative regulation of immature T cell proliferation in the thymus; negative regulation of signal transduction; negative regulation of T cell differentiation in the thymus; neuron development; ossification; osteoblast differentiation; pancreas development; pattern specification process; patterning of blood vessels; positive regulation of alpha-beta T cell differentiation; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of collagen biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; positive regulation of T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; proteoglycan metabolic process; regulation of cell differentiation; regulation of growth; skeletal development; smooth muscle development; smoothened signaling pathway; vasculature development; vitelline membrane formation
UniProt Protein Details:
NCBI Summary:
UniProt Code:P97812
NCBI GenInfo Identifier:6166227
NCBI Gene ID:16147
NCBI Accession:P97812.2
UniProt Secondary Accession:P97812,Q61724
UniProt Related Accession:P97812
Molecular Weight:45,485 Da
NCBI Full Name:Indian hedgehog protein
NCBI Synonym Full Names:Indian hedgehog
NCBI Official Symbol:Ihh��
NCBI Official Synonym Symbols:HHG-2��
NCBI Protein Information:indian hedgehog protein
UniProt Protein Name:Indian hedgehog protein
UniProt Synonym Protein Names:HHG-2
Protein Family:Indian hedgehog protein
UniProt Gene Name:Ihh��
UniProt Entry Name:IHH_MOUSE
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