Mouse HSP27 Recombinant Protein (RPPB5237)

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RPPB5237

Description

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Product Name:Mouse HSP27 Recombinant Protein
Product Code:RPPB5237
Size:10µg
Species:Mouse
Target:HSP27
Synonyms:HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The HSP27 protein solution (1mg/ml) contains 20mM Tris-HCl (pH8.0), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMTERRVP FSLLRSPSWE PFRDWYPAHS RLFDQAFGVP RLPDEWSQWF SAAGWPGYVR PLPAATAEGP AAVTLAAPAF SRALNRQLSS GVSEIRQTAD RWRVSLDVNH FAPEELTVKT KEGVVEITGK HEERQDEHGY ISRCFTRKYT LPPGVDPTLV SSSLSPEGTL TVEAPLPKAV TQSAEITIPV TFEARAQIGG PEAGKSEQSG AK

HSP27 or Estrogen-Regulated 24K proteinis a part of the mammalian small heat shock protein family. The protein is expressed constitutively in many tissues and its expression is increased to high levels after different types of stress including high temperatures, toxic metals, drugs and oxidants. Furthermore, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).

HSP27 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-209 a.a) and having a molecular mass of 25.4kDa. HSP27 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Chaperone; Heat shock protein

Cellular Component: axon; contractile fiber; cytoplasm; cytoskeleton; dendrite; extracellular space; focal adhesion; I band; intracellular; M band; nucleus; perikaryon; plasma membrane; proteasome complex; synapse; Z disc

Molecular Function:identical protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding

Biological Process: negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; response to unfolded protein

UniProt Code:P14602
NCBI GenInfo Identifier:547679
NCBI Gene ID:15507
NCBI Accession:P14602.3
UniProt Related Accession:P14602
Molecular Weight:19,380 Da
NCBI Full Name:Heat shock protein beta-1
NCBI Synonym Full Names:heat shock protein 1
NCBI Official Symbol:Hspb1��
NCBI Official Synonym Symbols:27kDa; Hsp25��
NCBI Protein Information:heat shock protein beta-1
UniProt Protein Name:Heat shock protein beta-1
UniProt Synonym Protein Names:Growth-related 25 kDa protein; Heat shock 25 kDa protein; HSP 25; Heat shock 27 kDa protein; HSP 27; p25
UniProt Gene Name:Hspb1��
UniProt Entry Name:HSPB1_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P14602
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