Mouse Immunology ELISA Kits
Mouse HMGB1 ELISA Kit
- SKU:
- MOFI00232
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P63158
- Sensitivity:
- 9.375pg/ml
- Range:
- 15.625-1000pg/ml
- ELISA Type:
- Sandwich
- Synonyms:
- Hmgb1, HMG-1, Amphoterin, high mobility group box 1, High mobility group protein 1, high-mobility group, nonhistone chromosomal protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydRate binding protein
- Reactivity:
- Mouse
- Research Area:
- Immunology
Description
Mouse HMGB1 ELISA Kit
HMGB1, a chromatin-binding protein and transcriptional regulator, acts as a damage associated molecular pattern (DAMP) when released extracellularly. It is a biomarker of inflammation, autoimmunity and cancers. The Assay Genie Mouse HMBG1 ELISA kit has been cited in various publications (view citations) whereby it is used as an indicator of active inflammation. It acts as a proinflammatory cytokine through binding to receptors TLR4 and RAGE and activating signaling pathways such as NF-κB and MAPK.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Mouse HMGB1 ELISA Kit |
| Product Code: | MOFI00232 |
| Size: | 96 Assays |
| Alias: | Hmgb1, HMG-1, Amphoterin, high mobility group box 1, High mobility group protein 1, high-mobility group, nonhistone chromosomal protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydRate binding protein |
| Detection Method: | Sandwich ELISA |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Mouse Hmgb1 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 9.375pg/ml |
| Range: | 15.625-1000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Mouse Hmgb1 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse Hmgb1 in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse Hmgb1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra Assay <8 Inter Assay <10 |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
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