Growth Factors & Cytokines Recombinant Proteins

Mouse HB-EGF Recombinant Protein (RPPB0270)

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SKU:
RPPB0270
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q06186
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse HB-EGF Recombinant Protein
Product Code:RPPB0270
Size:50µg
Species:Mouse
Target:HB-EGF
Synonyms:DTR, HEGFL, diphtheria toxin receptor, DTSF.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was filtered (0.2�m) and lyophilized from a concentrated solution containing 10mM PB and 500mM NaCl, pH7.4.
Solubility:It is recommended to reconstitute the lyophilized Mouse HB-EGF in sterile 18M-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Mouse HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL
Biological Activity:The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0�106 units/mg.

HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF�s ability to explicitly bind sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.

HB-EGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids (63-148 a.a.) and having a molecular mass of 9.8 kDa.The HB-EGF is purified by proprietary chromatographic techniques

UniProt Protein Function:HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function:heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein kinase B signaling cascade; cell migration; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; angiogenesis; blastocyst growth; positive regulation of cell growth; regulation of heart contraction; positive regulation of cell migration; wound healing, spreading of epidermal cells

UniProt Code:Q06186
NCBI GenInfo Identifier:1236400
NCBI Gene ID:15200
NCBI Accession:AAC52617.1
UniProt Related Accession:Q06186
Molecular Weight:22,808 Da
NCBI Full Name:heparin-binding epidermal growth factor-like growth factor
NCBI Synonym Full Names:heparin-binding EGF-like growth factor
NCBI Official Symbol:Hbegf��
NCBI Official Synonym Symbols:Dtr; Dts; Hegfl; AW047313��
NCBI Protein Information:proheparin-binding EGF-like growth factor; diphtheria toxin receptor; heparin binding epidermal growth factor-like growth factor
UniProt Protein Name:Proheparin-binding EGF-like growth factor
Protein Family:Proheparin-binding EGF-like growth factor
UniProt Gene Name:Hbegf��
UniProt Entry Name:HBEGF_MOUSE
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