Mouse Immunology ELISA Kits

Mouse GR beta / Glucocorticoid Receptor Beta ELISA Kit

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SKU:
MOFI00869
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06537-5
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
GRBeta
Reactivity:
Mouse

Description

Mouse GR beta / Glucocorticoid Receptor Beta ELISA Kit

GRβ is a splice variant of the glucocorticoid receptor gene that has been shown to have intrinsic activities and modulate gene expression and physiological functions in a cell-specific manner. The Assay Genie Mouse GR beta ELISA kit enables accurate measurement of GRβ levels, providing valuable insights into its role in cellular and physiological processes.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse GR beta / Glucocorticoid Receptor Beta ELISA Kit

Product Code:

MOFI00869

Size:

96 Assays

Alias:

GRbeta

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse GRbeta concentrations in serum plasma and other biological fluids.

Sensitivity:

9.375pg/ml

Range:

15.625-1000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse GRbeta and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse GRbeta in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

86-105

95

EDTA plasma (n=5)

89-99

93

UFH plasma (n=5)

86-103

92

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse GRbeta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

86-100%

87-105%

89-105%

EDTA plasma (n=5)

84-99%

85-99%

86-95%

UFH Plasma (n=5)

80-94%

85-99%

86-97%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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