Enzymes Recombinant Proteins

Mouse GPT2 Recombinant Protein (RPPB1742)

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RPPB1742

Description

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Product Name:Mouse GPT2 Recombinant Protein
Product Code:RPPB1742
Size:10µg
Species:Mouse
Target:GPT2
Synonyms:ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GPT2 solution (1 mg/ml) contains 2mM DTT, 20% Glycerol and 20mM Tris-HCl (pH7.5).
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than�85.0%�as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPERSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHAMGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIREDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSAVISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKCIEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSKGYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQFSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMAPDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS
Biological Activity:Specific activity is > 50 units/mg. Measured by the amount of enzyme that cleaves 1umole of L-Alanineto L-Glutamate per minute at pH7.5 at 37C?.

Glutamic-Pyruvate Transaminase 2 (GPT2) catalyzes the reversible transamination among alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT2 expressed mainly in muscle, fat and kidney and participates in the intermediary metabolism of glucose and amino acids.

GPT2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 542 amino acids (1-522 aa) and having a molecular mass of 60.1kDa.GPT2 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 2.6.1.2; TransferaseCellular Component: mitochondrionMolecular Function: transferase activity; transaminase activity; catalytic activity; alanine transaminase activity; pyridoxal phosphate bindingBiological Process: biosynthetic process; L-alanine metabolic process; 2-oxoglutarate metabolic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q8BGT5
NCBI GenInfo Identifier:27805389
NCBI Gene ID:108682
NCBI Accession:NP_776291.1
UniProt Secondary Accession:Q8BGT5,Q8BVY7, Q8K1J3
UniProt Related Accession:Q8BGT5
Molecular Weight:30.6kDa
NCBI Full Name:alanine aminotransferase 2
NCBI Synonym Full Names:glutamic pyruvate transaminase (alanine aminotransferase) 2
NCBI Official Symbol:Gpt2��
NCBI Official Synonym Symbols:ALT2; C87201; AU014768; AU041193; 4631422C05Rik��
NCBI Protein Information:alanine aminotransferase 2; GPT 2; glutamic--alanine transaminase 2; glutamic--pyruvic transaminase 2; glutamate pyruvate transaminase 2
UniProt Protein Name:Alanine aminotransferase 2
UniProt Synonym Protein Names:Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2
Protein Family:Acyl-acyl carrier protein thioesterase
UniProt Gene Name:Gpt2��
UniProt Entry Name:ALAT2_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q8BGT5
Research Area:
Enzymes
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