Description
| Product Name: | Mouse GPT2 Recombinant Protein |
| Product Code: | RPPB1742 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | GPT2 |
| Synonyms: | ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GPT2 solution (1 mg/ml) contains 2mM DTT, 20% Glycerol and 20mM Tris-HCl (pH7.5). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than�85.0%�as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPERSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHAMGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIREDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSAVISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKCIEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSKGYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQFSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMAPDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS |
| Biological Activity: | Specific activity is > 50 units/mg. Measured by the amount of enzyme that cleaves 1umole of L-Alanineto L-Glutamate per minute at pH7.5 at 37C?. |
Glutamic-Pyruvate Transaminase 2 (GPT2) catalyzes the reversible transamination among alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT2 expressed mainly in muscle, fat and kidney and participates in the intermediary metabolism of glucose and amino acids.
GPT2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 542 amino acids (1-522 aa) and having a molecular mass of 60.1kDa.GPT2 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 2.6.1.2; TransferaseCellular Component: mitochondrionMolecular Function: transferase activity; transaminase activity; catalytic activity; alanine transaminase activity; pyridoxal phosphate bindingBiological Process: biosynthetic process; L-alanine metabolic process; 2-oxoglutarate metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8BGT5 |
| NCBI GenInfo Identifier: | 27805389 |
| NCBI Gene ID: | 108682 |
| NCBI Accession: | NP_776291.1 |
| UniProt Secondary Accession: | Q8BGT5,Q8BVY7, Q8K1J3 |
| UniProt Related Accession: | Q8BGT5 |
| Molecular Weight: | 30.6kDa |
| NCBI Full Name: | alanine aminotransferase 2 |
| NCBI Synonym Full Names: | glutamic pyruvate transaminase (alanine aminotransferase) 2 |
| NCBI Official Symbol: | Gpt2�� |
| NCBI Official Synonym Symbols: | ALT2; C87201; AU014768; AU041193; 4631422C05Rik�� |
| NCBI Protein Information: | alanine aminotransferase 2; GPT 2; glutamic--alanine transaminase 2; glutamic--pyruvic transaminase 2; glutamate pyruvate transaminase 2 |
| UniProt Protein Name: | Alanine aminotransferase 2 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2 |
| Protein Family: | Acyl-acyl carrier protein thioesterase |
| UniProt Gene Name: | Gpt2�� |
| UniProt Entry Name: | ALAT2_MOUSE |
